How To Test Samples For H. Pylori

H. pylori is an antibody that can cause a variety of problems for humans. It can lead to peptic ulcers, chronic gastritis, and can also cause duodenal ulcers. It can also be part of the development of adenocarcinoma or even low-grade lymphoma of the mucosa, which is associated with lymphoid tissues of the stomach. Therefore, it is essential that you test your samples for this antibody. As such, you can use this antibody for research but shouldn’t use it for diagnostics.

More Information

This antibody has a clone called SPM526, and the immunogen is the purified Helicobacter pylori. It has an isotype called IgG1/k with an undetermined epitope.

Applications

The H. pylori test can be conducted using the Immunohistochemistry (IHC) application only. You should prepare the specimens using paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides are required, and you can clean them using graded alcohols and xylene or an alternative to xylene.

In most cases, you will need to dilute the concentrate formulation using a ratio of 1:100. If your dilutions are the same throughout testing protocols, you can also find a pre-diluted formula that is highly similar and will take less time to prepare the tests.

Once prepared, you will need to boil the tissue sections using a 10mM Citrate buffer with a pH of 6.0 for about ten to 20 minutes, allowing it to cool to room temperature for 20 minutes. This is the antigen retrieval step. You will also be required to incubate for 30 minutes while at room temperature.

The positive control is any Helicobacter pylori-infected stomach, and cellular localization will occur in the cell wall of the H. pylori.

The H. pylori test can help you determine which samples have this antibody present and which do not. Visit Spring Bioscience today to learn more and make your purchase.

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